Fig. 4

Effects of CsA on IFNγ signalling pathway proteins SOCS1 and pSTAT1. Protein levels of SOCS1, pSTAT1 and total STAT1 were measured by western blot in MSC stimulated with/out IFNγ (50 ng/ml) for at least 24 h followed by exposure to CsA (1000 ng/ml) for 1 h, 6 h or 24 h for the remainder of a 48 h window. All cells were harvested at a 48 h time-point. β-Actin was included as a loading control. Densitometry of n = 3 studies is shown for SOCS1 (b), pSTAT1 (c) and total STAT1 (d). Statistical analysis was carried out using unpaired Student’s t test where * < 0.05 and ** < 0.01